Shamsi M, Sedaghatkish A, Dejam M, Saghafian M, Mohammadi M, Sanati-Nezhad A. (A) The colonic mRNA expression of TNF- and IL-10 was analyzed by RT-PCR; (B) cytokine contents in colonic protein extracts were determined by ELISA; n=6 mice/group; n=3 for the control group (C) Serum TSG-6 level in different groups measured by ELISA. . If your cancer spreads to your abdomen or peritoneal space, IP chemotherapy can help target the cancer cells there. After 3 passages, spindle-shaped cell colonies gradually predominate in the cultures. Google Scholar. Injections, also known as shots, deliver liquid medications, fluids, or nutrients directly into a person's body. However, the therapeutic impacts of cell delivery route that is critical for clinical translation are currently poorly understood. It is more often applied to animals than to humans. Other than preformed at the site of injury, MSCs has showed ability to exert their therapeutic effects distally. . Very few reports pay attention to factors applied in the clinic, such as injection or exposure routes, which has highly impact on stem cell therapeutic efficiency for IBD12. Li, Y. P. et al. some potent chemo and radio-labeled drugs)59. Gastrointestinal Absorption of Insulin-like Growth Factor Note where each injection is made and subsequent injections should be made in slightly different spot than the previous injection. [13] The patient was a 51-year-old male who was hospitalized. Sci Rep 5, 16760, doi: 10.1038/srep16760 (2015). The excitation/ emission spectrum of DiR is in the near infrared range (excitation 750nm and emission 782nm). Intraperitoneal injection of 0.5-14.6 mg/kg 17- or 37-nm gold to mice produced an increase of brain dopamine, . Intraperitoneal Chemotherapy - Memorial Sloan Kettering Cancer Center -Lactalbumin is an example of a "retired" self-protein because its expression in normal . Gut epithelium is intact and lacks cytokines or chemokines attraction; 2) most of PCs are immune cells, such as B cells (5060%), macrophages and mast cells (30%) and T cells (510%)28. Intraperitoneal drug therapy: an advantage - PubMed Intraperitoneal but not intravenous cryopreserved mesenchymal stromal cells home to the inflamed colon and ameliorate experimental colitis. Intraperitoneal (IP) route of drug administration in laboratory animals is a common practice in many in vivo studies of disease models. (B) Quantification of FoxP3+ cells, the IP>IA and IV>DSS control, n=6 mice/group; n=3 for the control group. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Wang, M., Liang, C., Hu, H. et al. In IV group, the colon homing cells were fewer, most of MSCs were trapped in MSCs were rapidly accumulate into the lung and mononuclear phagocytic system. Intraperitoneal injections may also allow too-rapid entry of bacteria or LPS into the blood. Horwitz, E. M. et al. [5] In 1967, the production of amnesia was studied through an injection of physostigmine. In our study, we tracked the GFP+ MSCs colon colonization, but our results also showed the short stay of MSCs after injection. Treatment of dextran sodium sulfate-induced experimental colitis by adoptive transfer of peritoneal cells. Fischer, U. M. et al. Dig Dis 28, 386394, doi: 10.1159/000320392 (2010). Taede et al. von Bahr, L. et al. Methods of euthanasia likely to elicit distress vocalizations or pheromones that other animals could hear or smell should be performed in another location, if transportation distress can be minimized. 2A). And the loss of MSCs in the colon may attribute to many reasons, such as, wash out, cell death, or even rejection via the innate immune system. P values of<0.05 were considered statistically significant. There is a little fluid in between the peritoneal lining and your organs. Sonazoid was utilized to aid with contrast-enhanced ultrasonography by enhancing the peritoneal and pleural cavities. Intraperitoneal foreign bodies such as suture material, clips, and mesh also contribute to adhesion formation. This site needs JavaScript to work properly. Article Fraguas-Snchez AI, Torres-Surez AI, Cohen M, Delie F, Bastida-Ruiz D, Yart L, Martin-Sabroso C, Fernndez-Carballido A. Pharmaceutics. The rate and amount of drug transfer in the peritoneum are dependent on several factors. Careers. & Dittel, B. N. Isolation of mouse peritoneal cavity cells. Best way for mesenchymal stem cells transplantation for colitis", "Intraperitoneal injections as an alternative method for micro-CT contrast enhanced detection of murine liver tumors", "Optimization of Intraperitoneal Injection Anesthesia in Mice: Drugs, Dosages, Adverse Effects, and Anesthesia Depth", "Intraperitoneal Injection Is Not Always a Suitable Alternative to Intravenous Injection for Radiotherapy", "Pharmacological principles of intraperitoneal and bidirectional chemotherapy", Transjugular intrahepatic portosystemic shunt, https://en.wikipedia.org/w/index.php?title=Intraperitoneal_injection&oldid=1142986299, Short description is different from Wikidata, Articles with unsourced statements from April 2015, Articles with unsourced statements from January 2022, Creative Commons Attribution-ShareAlike License 3.0, This page was last edited on 5 March 2023, at 09:43. The https:// ensures that you are connecting to the The good cell-cell cross talk between MSCs and immune cells may be another contributor for better therapeutic effects. Tuning the Physicochemical Characteristics of Particle-Based Carriers for Intraperitoneal Local Chemotherapy. RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Bio Rad, Hercules, CA). In consistent with Scaldaferris work, the increased TSG-6 after MSCs transplantation was also identified. Intraperitoneal injection carries the risk of causing internal injury or of misplaced injection into a blood vessel or an organ, particularly the large thin-walled cecum or urinary bladder. Google Scholar. Collagen Deposition was assessed with phosphomolibidic acid-picrosirius red staining. Comparison of colonic FoxP3 expression 7 days after MSCs injection. Analysis of tissues following mesenchymal stromal cell therapy in humans indicates limited long-term engraftment and no ectopic tissue formation. On the contrary, the IP and AI groups showed more engraftment cells at the inflamed colon but showed fewer trapped cells in lung, liver and spleen (Fig. Intraperitoneal Injection of Oncolytic Viruses H101 for Patients With Refractory Malignant Ascites. The Ki-67+ cells were greatly increased after MSCs treatment. PubMed Central and K.W. The site is secure. The serum concentration of TNFinduced protein 6 (TSG-6) was measured in DSS colitic mice at 48 and 72hours after different routes of MSCs administrations. Cell stem cell 5, 5463, doi: 10.1016/j.stem.2009.05.003 (2009). These findings could be advanced by studying how the route of injection affects the organs in the peritoneal cavity. A modified human recombinant type 5 adenovirus . For some chemotherapy, such as ovarian cancer, IP injection is used to attempt to localize the drug . In various preclinical models, saroglitazar has been shown to prevent & reverse symptoms of NASH. IP injections are used for a variety of reasons: The peritoneum provides a large surface area for absorption of drug (compared with intramuscular (IM) or subcutaneous); can thus inject a larger fluid volume. Bioengineered. Overall, many studies utilize IP injections to deliver therapeutics to lab animals due to the efficiency of the administration route. Intraperitoneal Route of Drug Administration: Should it Be Used in . Intraperitoneal chemotherapy for peritoneal metastases: an expert opinion. Fresh feces from animals were collected for occult blood test, using fecal occult blood kit (Baso Diagnostics Inc., Zhuhai, China) according to the manufactures recommendations. The expression level of TNF-, the most important pro-inflammatory cytokine, was markedly decreased in IP group, compared with AI (P=0.0174), IV (P=0.0019) and control group (P<0.001), respectively. Your peritoneal (payr-ih-toh-NEE-ul) space is the area between the muscles and organs in your abdomen (belly). ADS Chemotactic cytokines might be involved and responsible for MSCs attraction. Pentobarbital sodium Injection Caution. When searching "intraperitoneal injections" a majority of the results relate to the use of IP injections in murine or rat models in order to study the effect of some drug or chemical. Rep. 6, 30696; doi: 10.1038/srep30696 (2016). Article Best way for mesenchymal stem cells transplantation for colitis. This study goes over the dosages, adverse effects, and more of using intraperitoneal injections of anesthesia.[18]. The few examples that do exist pertain to the treatment of pancreatic/ovarian cancers and injections of other drugs in clinical trials. IHC was performed according to the standard procedures. Upon intravenous and intraperitoneal injection of stressed MSA, fluorescent "hotspots" were observed in the spleens, livers and lungs. Truncal blocks and neuraxial . Google Scholar. In contrast, the DSS control showed 15.1% Ki-67+ cells/crypt. Most importantly, IP was found to have highest serum TSG-6 at both 48h and 72h and showed statistical significance when comparing with AI and DSS control at 72h. These findings, to some extent, may help to understand why IP is better than AI and IV. Injection of substances into peritoneum (body cavity), Current Drugs Delivered Intraperitoneally, "Why i.p. PDF An improved mouse model of sepsis based on intraperitoneal injections 81421003 and No. After 5 days of DSS contained water, all the subject mice showed strong positive test of fecal occult blood. Google Scholar. The results of this IP injection were the disappearance of the ascites associated with the rupture. Adhesions are a leading cause of secondary infertility in women, accounting for 15% to 20% of cases. Qualitatively, the existence of non-portal drainage was demonstrated by . of the needle exposed to assure 4-5mm injection depth. 5C). Cite this article. Cell stem cell 9, 1115, doi: 10.1016/j.stem.2011.06.008 (2011). 7 . Article 107vs. 109 ; Fig. However, they found the migrated PCs was very few and these may attribute to: 1) the recipient are heathy rat. The IP injection showed longer colon, with mean length of 8.24cm compared with 6.90cm in AI group (P=0.0057) and 6.14cm in IV group (P<0.001; Fig. . In patients with peritoneal surface malignancies, perioperative IP chemotherapy has been used with good results. and JavaScript. Injections Intraperitoneal (IP) "Mouse" Once animal is restrained turn over so abdomen is exposed. Radiation Protection of Polydatin Against Radon Exposure Injury of (B) MSCs signal quantification of five main organs (lung, liver, spleen, kidney and large bowel) and corresponding MLNs. Overall, this section provides a few examples of the effects and uses of intraperitoneal injections in human patients. 5B). Histopathological alterations in the mouse brain were detected after . Most recently published study by Scaldaferri et al. Article The complications associated with IP injection are major concern. official website and that any information you provide is encrypted Easier to inject that intravenous. Gastroenterology 114, 433440 (1998). This case is a good example of how IP injections can be used to deliver a drug that can help to treat or cure a medical diagnosis over the use of other routes of delivery. The Journal of infectious diseases 198, 456464, doi: 10.1086/590112 (2008). We even tried 1108 cell/mouse in IP and AI group and no immediate death was occurred. Supplies: However, most of these studies were focus on its therapeutic effects or mechanisms. Ray, A. Nature medicine 18, 618623, doi: 10.1038/nm.2695 (2012). In this study, the therapeutic efficiency of three different MSCs delivery routes (IP, AI and IV) were compared on DSS-induced colitis mouse model. A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone. Multiple Intravenous Transplantations of Mesenchymal Stem Cells Yu-Lung Chang, Huei-Yu Lo, Chin-Kan Chan, Xing-hua Pan, Qing-qing Li, Guang-ping Ruan, Hongsheng Yang, Rui Feng, Shenghong Zhang, H. R. Wardill, C. E. M. de Mooij, N. M. A. Blijlevens, Suze A. Jansen, Edward E. S. Nieuwenhuis, Caroline A. Lindemans, Scientific Reports We used SCID mice over nude mice for the metastatic survival model because the survival effects were more reproducibly obtained in SCID mice than in nude . Lu, Z., Wang, J., Wientjes, M. G. & Au, J. L. Intraperitoneal therapy for peritoneal cancer. The labeled cells were twice with warm fresh medium at 1500rpm for 5min to ensure complete removal of any unbound dye. Haematologica 93, 14571465, doi: 10.3324/haematol.12553 (2008). The IP 'option' was not adopted, however, because the standard treatment had shifted to IV cisplatin plus . 4F,G). Direct injection of sodium pentobarbital (referred to as euthanasia by . Choi, H., Lee, R. H., Bazhanov, N., Oh, J. Y. We also evaluated serum TSG-6 in three MSCs administration route. Evid Based Complement Alternat Med. Intraperitoneal Drug Delivery for Ovarian Cancer: Why, How, Who, What Quantification of collagen fiber was assessed using Image Pro Plus software (version 6.0). Human peripheral blood lymphocytes (hu-PBL) can be adoptively transferred by intraperitoneal injection into mice with severe combined immunodeficiency (SCID). These complications include catheter infection, physical damages to intra-abdominal structures, such as vaginal vault perforation, bladder erosion and bowel perforation58 and direct drug toxicity to non-specific abdomen organ (e.g. Further control study comparing bowel signals on healthy mice and DSS mice after MSCs injection showed weaker NIR signal on healthy bowel in all three routes, which indicated fewer MSCs migration to the colon without colitis (Supplementary Figure S5). [20] There needs to be more research done to determine appropriate dosing and combinations of these drugs to advance intraperitoneal drug delivery. Abstract Intraperitoneal (IP) injection is frequently reported to be as effective as intravenous (IV) injection. Unable to load your collection due to an error, Unable to load your delegates due to an error. It is more often applied to animals than to humans. 4 The scalp was disinfected and a midline incision was made in the skin. Since the IP injection induced more cells migration to the inflamed colon, we further compared the difference in mucosa healing of different delivery routes. Ankrum, J. Intraperitoneal injection - Wikipedia Federal government websites often end in .gov or .mil. The data showed that the TSG-6 levels increased with time in both IP and IV injection groups, while in DSS control group, the TSG-6 level decreased from 48h to 72h. Although there is no significant difference among three injection groups at 48h after MSCs transplantation, the serum TSG-6 was significant higher in IP injection group compared with naive group (P=0.024). The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. Disease recovery was evaluated by histological server score, gross body weight and survival rate. Cell stem cell 4, 206216, doi: 10.1016/j.stem.2009.02.001 (2009). Here, we also compare the expression of FoxP3, which is frequently used to quantitate Treg cells. Intraperitoneal Injection - an overview | ScienceDirect Topics The authors give their special thanks to the Dr. Yingchun Jia and Zhen Chen for their help in histological evaluation. ADS For example, if using a dose of 1 U kg -1 , a concentration of 0.2 U ml -1 is suitable. Intramuscular. Scientific Reports (Sci Rep) Skin application of non-rodents (miniature pigs) This shows an important limitation to the use of IP therapy. CAS Clinical and experimental immunology 116, 238245 (1999). Li, C. Y. et al. Depletion of O6-alkylguanine-DNA alkyltransferase activity in mammalian 2014ZX09508002). CAS Therapeutic strategies should be based on a sound and thorough understanding of the disease mechanisms, if possible, however the causes of IBD are still unclear6. Since that time, the literatures using stem cells for IBD have expanded. GAPDH mRNA was used as an internal control to normalize mRNA expression. Gonzalez, M. A., Gonzalez-Rey, E., Rico, L., Buscher, D. & Delgado, M. Adipose-derived mesenchymal stem cells alleviate experimental colitis by inhibiting inflammatory and autoimmune responses. showed that incubation of human monoclonal IgG aggregates in plasma for 24 hrs resulted in alteration of the total number of aggregates, led to different aggregate size and changed their . PubMed We selected the day 3 as observation time point. In that same year, a study injected egg whites intraperitoneally into rats to study changes in "droplet" fractions in kidney cells. Not only in animal studies, some clinical trials using MSCs to treat osteogensis imperfect (OI) in GvHD, also showed less than 1% cell were detected in target organ18,19,20. n=68 mice/group; n=3 for the control and naive (health mice) group. Comparison of colonic inflammatory cytokine TNF- and IL-10 at day 3. Aliment Pharmacol Ther 32, 939952, doi: 10.1111/j.1365-2036.2010.04439.x (2010). This case presents the question of how THC is absorbed in the peritoneal cavity. Patients should be anesthetized. Frontiers | Efficacy of intratympanic or postauricular subperiosteal Cardiovascular research 96, 208209; discussion 210203, doi: 10.1093/cvr/cvs202 (2012). Increased initial cell dose ensures an increased number of cells that reach to the injury site. It is estimated that as many as 1.4million Americans and 2.4million Europeans are suffering from these diseases1,2. Liotta, F. et al. 2A), which guarantee the less interference from the background signal that coming from free dye. PubMed Intraperitoneal (IP) Injection (Injection of Sodium Pentobarbital . Pulmonary passage is a major obstacle for intravenous stem cell delivery: the pulmonary first-pass effect. These mice were then randomized into 3 groups receiving different MSCs giving therapies. Evid Based Complement Alternat Med. 1B). In a previous study, 14 mice were exposed to SWCNTs through intravenous (IV) injection to determine whether there were any adverse effects caused by SWCNTs on the mouse brain and also to find a safe application dosage if possible. [1] Fluids are injected intraperitoneally in infants, also used for peritoneal dialysis. After intraperitoneal injections, 30% of the female mice died, compared with 3% of the male. FoxP3+ cells were greatly increased in MSCs therapy. Streptozotocin-induced Diabetic Models In Mice And Rats Sci. Clinical use of bone marrow, bone marrow concentrate and expanded bone marrow mesenchymal stem cells in cartilage disease. PDF UBC ANIMAL CARE COMMITTEE TECH 10a Intraperitoneal Injection in the Your peritoneal space. The peritoneum is a cavity which has been successfully utilized by nephrologists to perform peritoneal dialysis (PD) in patients with renal failure. Ko, I. K. et al. Goncalves Fda, C. et al. But this study at least pointed out the possible migration route for cells by intraperitoneal injection. Acute colitis was induced in C57BL/6 male mice by feeding 5% DSS (molecular weight 40000Da; Sigma) dissolved water for 5 days, followed by five days of regular drinking water according to our previously published study63. Sirius red staining showed disorganized collagen fibers in DSS control colon, which were diffusely distributed in the injured colon wall. New chapters on interventional . Pharm Res. In view of these observations, and the fact that NASH is a evaluated the therapeutic efficacy of different MSCs sources and delivery routes in experimental emphysema37. Internet Explorer). Additionally, the clinical translation is another concern. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Biological Sciences, China Agricultural University, Yuanmingyuan West Road No. The medium was refreshed every 23 days and third- and fourth-passage cells were used for all experiments.

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